| 質粒名稱: | pCuo DN Rac1 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 84644 |
| 存儲實驗室: | Sanjay Kumar |
|
載體骨架基本信息 |
|
| 載體名稱: | QM516B-1 |
| 載體出品公司: | System Biosciences |
| 載體類型: | 哺乳細胞表達, Lentiviral |
| 篩選標記: | Puromycin ; RFP |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | Rac1 |
| 物種來源: | H. sapiens (human) |
| 基因突變: | Dominant Negative |
| GenBank編號 | JN247409 |
| 啟動子: | cumate-inducible promoter |
| 克隆方法: | 未知 |
| 5’克隆位點: | CACCTGGCCCGATCTGGCC (from SparQ Cumate Switch System Biosciences manual) |
|
文獻引用方法 |
|
| 材料和方法部分: | pCuo DN Rac1 was a gift from Sanjay Kumar (Addgene plasmid # 84644 ; //n2t.net/addgene:84644 ; RRID:Addgene_84644) |
| 參考文獻部分: | Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters. MacKay JL, Kumar S. Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d. 10.1039/c4ib00099d PubMed 25044255 |
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